Search results for " Microsatellites"
showing 10 items of 32 documents
Isolation of seven polymorphic microsatellites in Ophioblennius atlanticus atlanticus (Perciformes, Blenniidae)
2005
We isolated and characterized seven polymorphic microsatellite loci of Ophioblennius atlanticus atlanticus (Valenciennes, 1836) using an optimized protocol to construct and screen a microsatellite-enriched genomic library. The analysis of variability was performed in 16 specimens from Faial Island (Azores, Portugal). The mean number of alleles was 8.71 ± ± ± 2.43 and the level of expected heterozygosity ranged from 0.764 to 0.903. The total exclusionary probabilities using these loci for the first and the second parent were 0.985 and 0.998, respectively, suggesting that these microsatellites are a useful tool for large-scale parentage analysis.
Genetic database development for the characterisation of Sicilian sheep population
2019
The most representative sheep in Sicily are Belicina, Comisana, Pinzirita, Barbarescaand the crossbred derived sheep from all this species. In this study, the allelic frequencies of the Sicilian sheep population were investigated. It currently represents the best way to determine the genetic identity and/or family even with limited amounts of sample or when the DNA is degraded. The aim of the study was to provide a reference data bank and to evaluate a microsatellite panel for pedigree analysis as suggested by the International Society for Animal Genetics (ISAG). There are various studies on European sheep, but few datasets were developed on the population of Sicilian sheep. The reference d…
Identification of (In)Compatible S-genotypes and Molecular Characterisation of Italian Sweet Cherry Cultivars.
2017
Italy is the first sweet cherry producer in Europe. Its rich germplasm of local varieties has not been fully characterised or exploited in breeding programmes. Sweet cherry is a self-incompatible species; this trait is controlled by a gametophytically expressed multi-allelic (S) locus. The knowledge of sweet cherry S-alleles and cross-incompatibility groups is important for growers and breeders for choosing appropriate pollinators in the orchard and planning crosses. In this work, we analysed 94 sweet cherry cultivars native of large part of the Italian regions where cherry is grown. The microsatellite primer pair set and the reference cultivars agreed for cherry by the Prunus Working Group…
Genetic structure of a patchily distributed philopatric migrant: implications for management and conservation
2017
Significant demographic fluctuations can have major genetic consequences in wild populations. The lesser kestrel (Falco naumanni) has suffered both population declines and range fragmentation during the second half of the 20th century. In this study we analysed multilocus microsatellite data to assess the genetic structure of the species. Our analysis revealed significant genetic structuring of lesser kestrel populations, not only at the cross-continental scale, but also regionally within the Central and Eastern (CE) Mediterranean region. We detected signs of genetic bottlenecks in some of the peripheral populations coupled with small effective population sizes. Values of genetic differenti…
Do island plant populations really have lower genetic variation than mainland populations? Effects of selection and distribution range on genetic div…
2015
Ecological and evolutionary studies largely assume that island populations display low levels of neutral genetic variation. However, this notion has only been formally tested in a few cases involving plant taxa, and the confounding effect of selection on genetic diversity (GD) estimates based on putatively neutral markers has typically been overlooked. Here, we generated nuclear microsatellite and plastid DNA sequence data in Periploca laevigata, a plant taxon with an island–mainland distribution area, to (i) investigate whether selection affects GD estimates of populations across contrasting habitats; and (ii) test the long-standing idea that island populations have lower GD than their mai…
Genetic and aromatic diversity of the Burgundy truffle
2013
Truffles are ectomycorrhizal Ascomycota fungi belonging to the Tuberaceae family and more specifically to the Tuber genus. More than thirty Tuber species are currently described in Europe. In this thesis, we specifically focused on the Tuber aestivum-uncinatum model. This truffle is commonly called "Burgundy Truffle" and has a gastronomic and cultural interest.The first part of this thesis focused on the taxonomic status of the Burgundy truffle (Tuber uncinatum). For this, we used a multi-marker approach combining several genetic markers commonly used at the interspecific scale. Our analyses showed that the two taxa, Tuber aestivum (summer truffle) and Tuber uncinatum are conspecific.In the…
Genetic diversity and clonal variation within the main Sicilian olive cultivars based on morphological traits and microsatellite markers
2014
The richness of Olea europaea (L.) genetic resources in Sicily is well documented. In the last 30 years, mostof the local cultivars, landraces and ecotypes have been gathered together in a large ex-situ collection,containing more than 300 genotypes. In this study, 45 putative clones of the main Sicilian olive cultivarswere characterized morphologically using microsatellite markers to unambiguously identify possiblesuperior genotypes. The microsatellites employed were polymorphic (observed heterozygosity = 0.71;polymorphic information content = 0.59), discriminated 52% of the genotypes and enabled the detectionof intra-cultivar polymorphism, derived from both somatic mutations, indicating th…
Genetic variation of an Italian long shelf-life tomato (Solanum lycopersicon L.) collection by using SSR and morphological fruit traits
2014
The recovery of ancient germplasm in tomato (Solanum lycopersicon L.) has become necessary to limit the wide genetic erosion caused by the employment of modern cultivars. Among germplasm collections, long shelf-life landraces could represent an important source of biodiversity. The present study provides a first set of molecular and phenotypic data on long shelf-life (so called "da serbo" in southern Italy) tomato collection, mainly originated from Sicily together with some landraces from Campania and Apulia. The analysis of fruit traits showed a low intra-varietal variation, while exhibiting a quite higher inter-varietal variability. Overall, the cultivars have been classified in six fruit…
An investigation of the self- and inter-incompatibility of the olive cultivars 'Arbequina' and 'Koroneiki' in the Mediterranean climate of Sicily
2016
In this investigation, the self-(in)compatibility of the Spanish cultivar Arbequina and the Greek cultivar Koroneiki was studied for the first time in Sicily, where these low vigour cultivars were recently introduced in super-intensive olive groves. Self- (S.P.) and openpollination (O.P.) tests, observation of fruit set and paternity test of seeds with microsatellite (SSR) markers, were performed to ascertain whether these cultivars were self-fertile and/or inter-compatible. For S.P. tests, branches with flowers at the balloon stage were bagged. For the O.P. tests, flowers were left to pollinate under natural conditions. Fruits from S.P. and O.P. were collected in November and fruit set was…
IL 10.G microsatellites mark promoter haplotypes associated with protection against the development of reactive arthritis in Finnish patients.
2001
Objective To investigate the association of microsatellites and single-nucleotide promoter polymorphisms (SNPs) in the gene for the cytokine interleukin-10 (IL-10) with susceptibility to and outcome of reactive arthritis (ReA). Methods From genomic DNA, IL-10 microsatellites G and R and IL-10 promoter polymorphisms at positions −1087 and −524 were typed by polymerase chain reaction, automated fragment length analysis, and restriction fragment digestion in 85 Finnish patients with ReA and 62 HLA–B27–positive Finnish controls. ReA patients had been followed up for 20 years. Genotypes and haplotypes of IL-10 were correlated with distinct features of the disease course, such as triggering agent…